Peb txoj kev tshawb fawb qhia tau hais tias Ganoderma lucidum / reishi / lingzhi qhia cov tshuaj tiv thaiv kab mob ntawm osteosarcoma hlwb hauv vitro.Nws tau pom tias Ganoderma lucidum inhibits qog nqaij hlav cancer ntawm lub mis loj hlob thiab kev tsiv teb tsaws los ntawm kev txwv Wnt / β-catenin signaling.Nws suppresses mob ntsws cancer los ntawm kev cuam tshuam ntawm focal adhesions thiab induction ntawm MDM2-mediated Slug degradation.Ganoderma lucidum inhibits cancer mis ntawm downregulating PI3K / AKT / mTOR txoj kev, Ganoderma lucidum plays lub luag hauj lwm antitumor nyob rau hauv mob leukemia hlwb los ntawm thaiv txoj kev MAPK.
CCK-8 thiab colony formation assays, rau kev ntsuam xyuas cov nyhuv ntawm Ganoderma lucidum ntawm osteosarcoma cell kab viability thiab proliferation, qhia tau hais tias Ganoderma lucidum suppresses proliferation ntawm MG63 thiab U2-OS hlwb nyob rau hauv ib lub sij hawm- thiab concentration-dependent yam, thiab thiaj li. muaj peev xwm ntawm cov cell kom colonize.
Ganoderma lucidum upregulates cov kev qhia ntawm proapoptotic noob, thiab ntws cytometry tsom xam pom tias apoptosis ntawm MG63 thiab U2-OS hlwb yog nce tom qab kho nrog Ganoderma lucidum.Kev tsiv teb tsaws ntawm tes yog lub hauv paus ntawm ntau yam kev coj cwj pwm lom neeg, suav nrog angiogenesis, kho qhov txhab, mob, thiab mob qog noj ntshav.Ganoderma lucidum txo qis kev tsiv teb tsaws thiab ntxeem tau ntawm ob kab ntawm tes thiab inhibits kev loj hlob, tsiv teb tsaws, thiab ntxeem tau, thiab induces apoptosis ntawm osteosarcoma hlwb.
Aberrant Wnt / β-catenin signaling yog ze ze rau kev tsim, metastasis, thiab apoptosis ntawm ntau hom qog noj ntshav, nrog rau kev tswj hwm ntawm Wnt / β-catenin signaling tau pom hauv osteosarcoma.
Hauv txoj kev tshawb no, dual-luciferase tus kws tshuaj ntsuam xyuas tau pom tias Ganoderma lucidum kho blocks CHIR-99021-activated Wnt/β-catenin signaling.Qhov no tau ua pov thawj ntxiv los ntawm peb qhov kev ua qauv qhia tias kev hloov pauv ntawm Wnt lub hom phiaj, xws li LRP5, β-catenin, cyclin D1, thiab MMP-9, yog inhibited thaum osteosarcoma hlwb raug kho nrog Ganoderma lucidum.
Cov kev tshawb fawb yav dhau los tau pom nyob rau hauv cov kev kuaj mob uas LRP5 yog upregulated nyob rau hauv osteosarcoma txheeb ze rau ib txwm cov ntaub so ntswg, thiab kev qhia ntawm LRP5 cuam tshuam nrog cov kab mob metastatic thiab cov kab mob tsis zoo, ua rau LRP5 yog lub hom phiaj kho mob rau osteosarcoma.
β-catenin nws tus kheej yog lub hom phiaj tseem ceeb hauv Wnt / β-catenin signaling txoj hauv kev, thiab kev nthuav qhia ntawm β-catenin hauv osteosarcoma yog nce ntxiv.Thaum β-catenin hloov mus rau hauv lub nucleus los ntawm cytoplasm, nws qhib kev qhia ntawm nws cov noob caj noob ces, uas suav nrog cyclin D1, C-Myc, thiab MMPs.
Myc yog ib qho ntawm cov proto-oncogenes loj thiab ua lub luag haujlwm tseem ceeb hauv kev tswj kev ua haujlwm, kev hloov pauv, thiab inhibition ntawm cov noob qhia. osteosarcoma.
Cyclin D1 yog lub voj voog ntawm tes tseem ceeb G1 theem regulator thiab accelerates G1 / S theem hloov.Kev qhia ntau dhau ntawm cyclin D1 tuaj yeem ua rau lub voj voog ntawm tes luv thiab txhawb kev loj hlob ntawm tes sai hauv ntau hom qog.
MMP-2 thiab MMP-9 yog stromelysins uas muaj peev xwm degrade extracellular matrix Cheebtsam, ib tug tseem ceeb feature rau qog angiogenesis thiab ntxeem tau.
Qhov no qhia tau hais tias Wnt / β-catenin lub hom phiaj cov noob ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm osteosarcoma, thiab tias thaiv cov teeb liab nodes yuav muaj txiaj ntsig zoo heev.
Tom qab ntawd, peb tau kuaj pom qhov kev qhia ntawm mRNA thiab cov protein ntawm Wnt / β-catenin signaling-txog cov hom phiaj ntawm PCR thiab western blotting.Hauv ob kab ntawm tes, Ganoderma lucidum inhibited kev qhia ntawm cov proteins thiab cov noob.Cov txiaj ntsig no qhia ntxiv tias Ganoderma lucidum inhibits Wnt / β-catenin signaling los ntawm kev tsom LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, thiab MMP-9.
E-cadherin yog transmembrane glycoprotein nthuav dav hauv cov hlwb epithelial thiab mediates adhesion ntawm epithelial hlwb thiab stromal hlwb.Kev tshem tawm lossis poob ntawm E-cadherin qhia ua rau poob lossis tsis muaj zog ntawm adhesion ntawm cov qog hlwb, ua rau cov qog hlwb txav tau yooj yim dua, thiab tom qab ntawd ua rau cov qog infiltrate, diffuse, thiab metastasize.Hauv txoj kev tshawb no, peb pom tias Ganoderma lucidum tuaj yeem tswj hwm E-cadherin, yog li tiv thaiv Wnt/β-catenin-mediated phenotype ntawm osteosarcoma hlwb.
Hauv kev xaus, peb cov txiaj ntsig tau qhia tias Ganoderma lucidum blocks osteosarcoma Wnt / β-catenin signaling thiab thaum kawg ua rau txo qis ntawm osteosarcoma cell kev ua.Cov kev tshawb pom no qhia tias Ganoderma lucidum yuav yog ib qho txiaj ntsig zoo thiab muaj txiaj ntsig zoo rau kev kho mob osteosarcoma, cov khoom lag luam muaj xws liGanoderma lucidum spore oil softgels/reishi spore oil softgels,Ganoderma lucidum spore hmoov / reishi spore hmoov
Lub sij hawm xa tuaj: Plaub Hlis-18-2022